Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons

Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons

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Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in Water Chamber Air Break dissociated cell culture.Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium.These cultures can be used for Filters morphological, biochemical and electrophysiological studies of neuronal growth and function.